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.Nucleofection is a specialized form of electroporation that enables the transfer of genetic material into a cell nucleus. Higher cell densities may cause lower Nucleofection Efficiencies Trypsinization 16 Remove media from the cultured cells and wash cells once with PBS.
Higher cell densities may cause lower nucleofection efficiencies. Collect the cells in a 15-ml conical tube. Plug the pipette into position in the Neon transfection device.
In addition nucleofection is a fast method because the results can be analyzed within 7 h.
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